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- W2377308915 abstract "OBJECTIVE To construct the recombinant plasmid of pET32a-UL12 and to compare the expression level and biological activity of expression products of pET32a-UL12 in E.coli BL21 and E.coli Rosetta.METHODS The UL12 gene of HSV-1 was obtained by PCR,and was cloned into pET32a(+).The recombinant plasmid of pET32a-UL12 was transformed into E.coli BL21 and E.coli Rosetta.The expressed proteins of the E.coli BL21 and E.coli Rosetta were identified by SDS-PAGE.Then,the expression level and the biological activity of the two proteins were compared in this study.RESULTS The UL12 gene was successfully expressed both in E.coli BL21 and in E.coli Rosetta.The UL12 gene expressed more proteins in E.coli Rosetta than that in E.coli BL21,while the biological activity of the proteins expressed in E.coli BL21 was higher than that in E.coli Rosetta.CONCLUSION The HSV-1 alkaline nuclease with high biological activity was obtained in the study which is very important in the research of anti-virus remedies." @default.
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- W2377308915 date "2012-01-01" @default.
- W2377308915 modified "2023-09-23" @default.
- W2377308915 title "The differential expression of HSV-1 UL12 gene in E.coli BL21 and E.coli Rosetta" @default.
- W2377308915 hasPublicationYear "2012" @default.
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