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- W2377310955 abstract "Objective: To research and make the agent for inhibiting activity of residual UDG in PCR products. Methods:DNA EIA technique was applied to detect the hybridization rates of all dU DNA PCR products containing stablizer and without stablizer, the hybridization rates at room temperature for 24 hours and at 37 ℃ for 48 hours. Results: (1)Stablizers A and B were added in the PCR products of which 5 μl was taken out for hybridization and their hybridization rates were 61.470%, 68.996% ,respectively. The hybridization rate of the control was 99.283%.(2) After 30 μl of the PCR products were added to stablizers, diluted to 70 μl with 1×PCR buffer ,and then kept at 37 ℃ for 48 hours 10μl of which was taken out for hybridization. Hybridization rates with 5 μl and 2.5 μl of stablizers A were 71.092% and 67.691%,respectively. The controls were 68.020% and 63.906%.The hybridization rates of the groups with stablizer B were 91.333% and 80.307%,The controls were 63.906% and 68.020%. Conclusion: In this study, the results of the hybridization rates with DNA EIA method revealed that hybridization of the PCR products which were not diluted and were directly added to the stablizer was inhibited .In contrast, hybridization of the PCR products added to the and diluted was not inhibited. After the mixture of PCR products and the stablizer were diluted, hybridization rates with stablizer A increased by 3.072% and 3.095% as compared with that of controls. Accordingly, hybridization rates of the groups with stablizer B increased by 27.427% and 12.287% as compared with that of controls. The results above indicate that stablizer B may inhibit the activity of residual UDG and play a central role in protecting all dU DNA PCR products." @default.
- W2377310955 created "2016-06-24" @default.
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- W2377310955 date "2002-01-01" @default.
- W2377310955 modified "2023-09-25" @default.
- W2377310955 title "Stablizer used in inhibiting residual uracil DNA glycosylase activity in the PCR products" @default.
- W2377310955 hasPublicationYear "2002" @default.
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