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- W2377375249 abstract "Several factors affecting the protoplasts formation of Sinorhizobium.Fredii with antagonistic to SCN and root rot of soybean were studied.The growth curve was determined and the optimum cell-age for protoplasts formation was in the logarithmic growth mid-phase,the maximum yield of protoplast was accelerated when pretreated by 0.5% penicillium in the culture medium,the optimum enzyme condition was digesting at 35℃ with 1.5 mg·mL-1lysozyme,the process of protoplasts release was observed using microscope.The foundation for genetic breeding of S.Fredii has been laid by using the protoplasts." @default.
- W2377375249 created "2016-06-24" @default.
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- W2377375249 date "2010-01-01" @default.
- W2377375249 modified "2023-09-23" @default.
- W2377375249 title "Protoplast Formation of Antagonistic Rhizobium" @default.
- W2377375249 hasPublicationYear "2010" @default.
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