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- W2377398869 abstract "The molecular structure of VP2 protein of canine parvovirus isolated from Nanjing area(CPV-GN)was predicted by bioinformatic software,from which a highly immunogenic fragment VP2'was screened and amplified by PCR.The PCR product was inserted into pMD 18-T vector and the resulting recombinant plasmid was double digested with EcoR I and Sal I.The expression vector pET32-VP2',designed to propagate and express the fusion protein in Escherichia coli BL21,was constructed by subcloning VP2' into pET32a(+)vector.The desired fusion protein with a molecular weight of 34 ku was detected by SDS-PAGE after induction by IPTG.Subsequent Western bloting assay showed that the recombinant protein could be recognized by the antibody against CPV-2b,and thus has retained the major epitopes of the native protein VP2." @default.
- W2377398869 created "2016-06-24" @default.
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- W2377398869 date "2008-01-01" @default.
- W2377398869 modified "2023-09-22" @default.
- W2377398869 title "Cloning and expression of the immunogenic fragment of VP2 protein of canine parvovirus isolated from Nanjing area" @default.
- W2377398869 hasPublicationYear "2008" @default.
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