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- W2377413117 abstract "Objective To identify the polioviruses firstly using real time fluorescent quantitative reverse transcription polymerase chain reaction(rRT- PCR)method in Hebei Provincial Polio Laboratory, to evaluate the assay and provide a basis for performing intratypic differentiation by conventional rRT- PCR in the polio laboratory. Methods According to rRT- PCR recommended by WHO, poliovirus isolates from the polio laboratory were tested for intratypic differentiation and vaccine derived polioviruses(VDPVs)screening. Results The results of intratypic differentiation of polioviruses using rRT- PCR were completely consistent with those of VP1 region sequencing. But the results of VDPV rRT- PCR for poliovirus did not completely consist with those of VP1 region sequencing. Two strains of type 2 poliovirus were misidentified as non- Sabin- like viruses,with the false positive rate of 6.9%(2/29). Conclusions The rRT- PCR method can be used for routine surveillance of poliovirus in Hebei Provincial Polio Laboratory." @default.
- W2377413117 created "2016-06-24" @default.
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- W2377413117 date "2013-01-01" @default.
- W2377413117 modified "2023-09-28" @default.
- W2377413117 title "Application of rRT-PCR Method in Intratypic Identification of Poliovirus in a Polio Laboratory" @default.
- W2377413117 hasPublicationYear "2013" @default.
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