Matches in SemOpenAlex for { <https://semopenalex.org/work/W2377435065> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2377435065 abstract "To evaluate the immunogenicity of galE mutant vaccine and immune protective efficacy against challenge with high-dose Campylobacter jejuni.BALB/c mice were immunized orally either with single dose of galE mutants in low and high dose or with two doses of galE mutants in low and high dose, respectively, and parental strains were orally administered to immunize mice serving as the positive control. ELISA was performed to examine the titers of CJ-specific secretory IgA and of CJ-specific IgG, in intestinal lavage at 7 days and in sera at 21 days post-immunization, respectively. At 26 days after immunization, the animals were challenged orally by CJ,in high dose (10~9CFU/ per mouse). The protective efficacies of galE mutant vaccine in mice, including protection against disease symptoms (by measuring the degrees of reducing illness indexes), against intestinal colonization (by culturing stool samples) were evaluated. The results showed that ① Significant enhanced titers of CJ-specific sIgA and IgG were detected in intestinal lavage at 7 days and in sera at 21 days, respectively, from the mice immunized by galE mutants whether with single dose in low dose and high dose or with two doses in low dose and high dose. No obvious differences were seen between with single dose and with two dose, as well as between with low dose and with high dose(P0.05). The difference in levels of the special antibodies was very significant between the mice immunized with galE mutants and the mice immunized with broth (negative control) (P0.01). No marked difference in inducing levels of sIgA and IgG by parental strains or by galE mutants(P0.05).② Animals immunized with galE mutants had significant low illness indexes after challenge by high-dose CJ(P0.01), and the protective efficacies of galE mutants against disease symptoms were 79.5%～83.1%. Animals immunized with galE mutants also showed marked reduction in intestinal colonization, and at 7 days after challenge, 80%-90% of the animals had been colonization negative. The differences in protective efficacies against disease symptoms and against intestinal colonization between galE mutants and parental strains, as well as between different doses of galE mutants, were not significant(P0.05).It is concluded that ① galE mutants remain similar immunogenicity and the immune protective efficacy to the parental strains, and could effectively induce CJ-specific sIgA and IgG antibodies responses in mice and galE mutants provide protective efficacy against high-dose CJ challenge for vaccinated mice that showed significant low illness indexes, marked reduction in colonization. ②By administered orally, with a single dose only in low dose, the galE mutants could provide a effective protective efficacy for vaccinated mice." @default.
- W2377435065 created "2016-06-24" @default.
- W2377435065 creator A5091772988 @default.
- W2377435065 date "2005-01-01" @default.
- W2377435065 modified "2023-09-23" @default.
- W2377435065 title "Immunogenicity and protective efficacy of galE mutant vaccine in mice" @default.
- W2377435065 hasPublicationYear "2005" @default.
- W2377435065 type Work @default.
- W2377435065 sameAs 2377435065 @default.
- W2377435065 citedByCount "0" @default.
- W2377435065 crossrefType "journal-article" @default.
- W2377435065 hasAuthorship W2377435065A5091772988 @default.
- W2377435065 hasConcept C104317684 @default.
- W2377435065 hasConcept C126838900 @default.
- W2377435065 hasConcept C134018914 @default.
- W2377435065 hasConcept C143065580 @default.
- W2377435065 hasConcept C149857219 @default.
- W2377435065 hasConcept C159654299 @default.
- W2377435065 hasConcept C203014093 @default.
- W2377435065 hasConcept C22889606 @default.
- W2377435065 hasConcept C2777863537 @default.
- W2377435065 hasConcept C2780801004 @default.
- W2377435065 hasConcept C2780868878 @default.
- W2377435065 hasConcept C3019987054 @default.
- W2377435065 hasConcept C32611913 @default.
- W2377435065 hasConcept C55493867 @default.
- W2377435065 hasConcept C71924100 @default.
- W2377435065 hasConcept C86803240 @default.
- W2377435065 hasConcept C8891405 @default.
- W2377435065 hasConcept C98274493 @default.
- W2377435065 hasConceptScore W2377435065C104317684 @default.
- W2377435065 hasConceptScore W2377435065C126838900 @default.
- W2377435065 hasConceptScore W2377435065C134018914 @default.
- W2377435065 hasConceptScore W2377435065C143065580 @default.
- W2377435065 hasConceptScore W2377435065C149857219 @default.
- W2377435065 hasConceptScore W2377435065C159654299 @default.
- W2377435065 hasConceptScore W2377435065C203014093 @default.
- W2377435065 hasConceptScore W2377435065C22889606 @default.
- W2377435065 hasConceptScore W2377435065C2777863537 @default.
- W2377435065 hasConceptScore W2377435065C2780801004 @default.
- W2377435065 hasConceptScore W2377435065C2780868878 @default.
- W2377435065 hasConceptScore W2377435065C3019987054 @default.
- W2377435065 hasConceptScore W2377435065C32611913 @default.
- W2377435065 hasConceptScore W2377435065C55493867 @default.
- W2377435065 hasConceptScore W2377435065C71924100 @default.
- W2377435065 hasConceptScore W2377435065C86803240 @default.
- W2377435065 hasConceptScore W2377435065C8891405 @default.
- W2377435065 hasConceptScore W2377435065C98274493 @default.
- W2377435065 hasLocation W23774350651 @default.
- W2377435065 hasOpenAccess W2377435065 @default.
- W2377435065 hasPrimaryLocation W23774350651 @default.
- W2377435065 hasRelatedWork W142878952 @default.
- W2377435065 hasRelatedWork W1778154044 @default.
- W2377435065 hasRelatedWork W1875934414 @default.
- W2377435065 hasRelatedWork W2087974772 @default.
- W2377435065 hasRelatedWork W2091561373 @default.
- W2377435065 hasRelatedWork W2100410890 @default.
- W2377435065 hasRelatedWork W2108828539 @default.
- W2377435065 hasRelatedWork W2137129186 @default.
- W2377435065 hasRelatedWork W2142898866 @default.
- W2377435065 hasRelatedWork W2155035342 @default.
- W2377435065 hasRelatedWork W2193055766 @default.
- W2377435065 hasRelatedWork W2351677845 @default.
- W2377435065 hasRelatedWork W2353674669 @default.
- W2377435065 hasRelatedWork W2354017400 @default.
- W2377435065 hasRelatedWork W2370145575 @default.
- W2377435065 hasRelatedWork W2373000903 @default.
- W2377435065 hasRelatedWork W2384560901 @default.
- W2377435065 hasRelatedWork W2466681485 @default.
- W2377435065 hasRelatedWork W3027092493 @default.
- W2377435065 hasRelatedWork W3212223346 @default.
- W2377435065 isParatext "false" @default.
- W2377435065 isRetracted "false" @default.
- W2377435065 magId "2377435065" @default.
- W2377435065 workType "article" @default.