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- W2377444162 abstract "Objective:To investigate the difference of expression level between classical eukaryotic expression system and eukaryonized prokaryotic expression system made of pCMV-T7pol and pT7IRES-HBs in eukaryotic cell environment.Methods:Eukaryonized prokaryotic expression plasmid was constructed and gene immunize was conducted with these plasmid;the antibody induced by these plasmid was evaluated by DOT-EIA and ELISA.Results:It was demonstrated that the eukaryonized prokaryotic expression system could efficiently enhance the expression level of target gene in eukaryotic cells. To further investigate the ability of eukaryonized prokaryotic expression system to induce HBV specific humoral immune response, mice were intramuscularly immunized with the mixture of the plasmid pCMV-T7pol and the plasmid pT7IRES-HBs. It was demonstrated that HBV specific humoral response not only could be induced, but also was much stronger than that induced by the classical eukaryotic expression system. And the HBV specific Th2 type immune response was greatly enhanced(P0.01).Conclusion:Eukaryonized prokaryotic expression system could induce stronger humoral response in vivo." @default.
- W2377444162 created "2016-06-24" @default.
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- W2377444162 date "2007-01-01" @default.
- W2377444162 modified "2023-09-25" @default.
- W2377444162 title "Enhancing effect of eukaryonized prokaryotic expression system on HBV preS2/S gene immunization" @default.
- W2377444162 hasPublicationYear "2007" @default.
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