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- W2377448291 abstract "Different concentrations of 5 azacytidine combining with different treatment time were employed to treat the transgenic rice with a transcriptionally silenced cry1Ab transgene. The results indicated that 5 azacytidine could reactivate 8%-30% of the silenced transgenic rice plants and the expression of the reactivated cry1Ab transgene could reach as high as 0.147% of the total soluble protein. Treatment with low concentration of 5 azacytidine could lead to the highest reactivation ratio and the expression of the cry1Ab transgene. Meanwhile, treatment with azacytidine could result in dwarfism, reduction in tiller numbers, grain weight per plant, panicle length and the delaying in flowering. The study showed that 5 azacytidine could be used as a potential mutagen in overcoming of transgene silencing and creation of the transgenic plants with elite agronomic traits." @default.
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- W2377448291 date "2001-01-01" @default.
- W2377448291 modified "2023-09-22" @default.
- W2377448291 title "5 azacytidine mediated Phase Reactivation of cry1Ab Transgene and Its Biological Effects on the Transgenic Rice" @default.
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