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- W2377459405 abstract "Objective:To study the effect of lipopolysaccharide(LPS)on glucose transporter-1(GLUT-1)expression in cultured human peritoneal mesothelial cell(HPMC).Methods:Enzymatic disaggregation was used for primary culture and passage of HPMC.Cytokeratin was determined by indirect immunofluorescence for HPMC identification.Immunohistochemistry(ABC)was used for GLUT-1 identification and semi-quantitative analysis.The expression of GLUT-1 mRNA was evaluated by reverse transcription polymerase chain reaction(RT-PCR)technology.Hexokinase assay was used for measurement of concentration of glucose in cell culture fluid,and then net glucose utilization was calculated.Results:(1)The result of indirect immunofluorescence showed that the antibody to cytokeratin of cultured HPMC dyeing presented green fluorescence and this is the character of HPMC.(2)LPS can stimiulate HPMC to increase the expression of GLUT-1 protein and mRNA and net glucose utilization significantly in normal glucose concentration(0.1%)in a concentration-and time-dependent manner(P0.05).At concentration of 100 μg/mL for 24 h,LPS made the expression of GLUT-1 protein and mRNA increase to the most in different concentrations and periods(P0.01).(3)At high concentration of glucose(4.25%),LPS can increase the expression of GLUT-1 protein and mRNA significantly compared to the high glucose group and the control group(P0.05).Conclusion:LPS can increase the GLUT-1 expression and net glucose utilization of HPMC,which displayed more significantly in high concentration glucose.This showed us a clue to study and prevention and cure the ultrafiltration failure of peritoneal dialysis in peritonitis." @default.
- W2377459405 created "2016-06-24" @default.
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- W2377459405 date "2007-01-01" @default.
- W2377459405 modified "2023-09-26" @default.
- W2377459405 title "Effects of Lipopolysaccharide on Glucose Transporter-1 Expression in Cultured Human Peritoneal Mesothelial Cell" @default.
- W2377459405 hasPublicationYear "2007" @default.
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