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- W2377492711 abstract "Objective To establish human skin keratinocytes bank in vitro . Methods Human keratinocytes were isolated, cultured, subcultured, stored in liquid nitrogen, and recovered utilized. The condition of growth after passage, recovery and viability were observed and identified by immunocytochemical (ABC) method. Results The keratinocytes were subcultured at least 3 passages with more than 1×10 12 over the original plating number. After frozen storage and recovery, the keratinocytes also proliferated and differentiated well, and showed similar growth behavior as primarily cultured keratinocytes. Conclusion The keratinocytes bank can be established by easy and feasible technique under routine laboratory culture conditions." @default.
- W2377492711 created "2016-06-24" @default.
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- W2377492711 date "2004-01-01" @default.
- W2377492711 modified "2023-09-25" @default.
- W2377492711 title "Construction of human skin keratinocytes bank" @default.
- W2377492711 hasPublicationYear "2004" @default.
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