Matches in SemOpenAlex for { <https://semopenalex.org/work/W2377579002> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2377579002 abstract "Objective To evaluate the inhibitory effect of tumor necrosis factor alpha (TNF-α) on proliferation and apoptosis of human cholangiocarcinoma cells.Methods Recombinant reverse transcript virus TNF-α gene was transfected into the cells of human cholangiocarcinoma in vitro,which stably overexpressed TNF-α gene.The expression of the gene in the transfected cells was detected by PCR and immunocytochemical methods respectively.The cycle pattern and apoptosis were assayed by using flow cytometry.Results After transfection of recombinant reverse transcript virus TNF-α gene,the cells growth rate were obviously inhibited and the cells arrested in G_1 phase,presenting that the proportion of the cells in G_1 phase was obviously increased from 29.07% to 54.24%,and that of the cells in S and G_2-M phases obviously decreased from 10.34% to 18.32% and from 52.03% to 2.02%,respectively,as compared with control group.Colony formation in these recombinant reverse transcript virus TNF-α-infected cells was greatly decreased as compared to the recombinant reverse transcript virus TNF-α-uninfected cells.The apoptotic index was increased in the recombinant reverse transcript virus TNF-α-infected cells from 5.83% to 34.76%.Conclusion Tranfer of TNF-α gene could inhibit the growth of human cholangiocarcinoma cells and induce cell apoptosis." @default.
- W2377579002 created "2016-06-24" @default.
- W2377579002 creator A5082831342 @default.
- W2377579002 date "2004-01-01" @default.
- W2377579002 modified "2023-09-23" @default.
- W2377579002 title "Effects of recombinant reverse transcript virus tumor necrosis factor alpha gene transduction on proliferation and apoptosis of human cholangiocarcinoma cells" @default.
- W2377579002 hasPublicationYear "2004" @default.
- W2377579002 type Work @default.
- W2377579002 sameAs 2377579002 @default.
- W2377579002 citedByCount "0" @default.
- W2377579002 crossrefType "journal-article" @default.
- W2377579002 hasAuthorship W2377579002A5082831342 @default.
- W2377579002 hasConcept C104317684 @default.
- W2377579002 hasConcept C153911025 @default.
- W2377579002 hasConcept C159047783 @default.
- W2377579002 hasConcept C17991360 @default.
- W2377579002 hasConcept C190283241 @default.
- W2377579002 hasConcept C203014093 @default.
- W2377579002 hasConcept C2522874641 @default.
- W2377579002 hasConcept C40767141 @default.
- W2377579002 hasConcept C54009773 @default.
- W2377579002 hasConcept C54355233 @default.
- W2377579002 hasConcept C553184892 @default.
- W2377579002 hasConcept C55493867 @default.
- W2377579002 hasConcept C81885089 @default.
- W2377579002 hasConcept C86803240 @default.
- W2377579002 hasConceptScore W2377579002C104317684 @default.
- W2377579002 hasConceptScore W2377579002C153911025 @default.
- W2377579002 hasConceptScore W2377579002C159047783 @default.
- W2377579002 hasConceptScore W2377579002C17991360 @default.
- W2377579002 hasConceptScore W2377579002C190283241 @default.
- W2377579002 hasConceptScore W2377579002C203014093 @default.
- W2377579002 hasConceptScore W2377579002C2522874641 @default.
- W2377579002 hasConceptScore W2377579002C40767141 @default.
- W2377579002 hasConceptScore W2377579002C54009773 @default.
- W2377579002 hasConceptScore W2377579002C54355233 @default.
- W2377579002 hasConceptScore W2377579002C553184892 @default.
- W2377579002 hasConceptScore W2377579002C55493867 @default.
- W2377579002 hasConceptScore W2377579002C81885089 @default.
- W2377579002 hasConceptScore W2377579002C86803240 @default.
- W2377579002 hasLocation W23775790021 @default.
- W2377579002 hasOpenAccess W2377579002 @default.
- W2377579002 hasPrimaryLocation W23775790021 @default.
- W2377579002 hasRelatedWork W1963603360 @default.
- W2377579002 hasRelatedWork W2018427162 @default.
- W2377579002 hasRelatedWork W2033836633 @default.
- W2377579002 hasRelatedWork W2236862998 @default.
- W2377579002 hasRelatedWork W2348045231 @default.
- W2377579002 hasRelatedWork W2348721063 @default.
- W2377579002 hasRelatedWork W2352563561 @default.
- W2377579002 hasRelatedWork W2355480600 @default.
- W2377579002 hasRelatedWork W2381461172 @default.
- W2377579002 hasRelatedWork W2383356758 @default.
- W2377579002 hasRelatedWork W2386016531 @default.
- W2377579002 hasRelatedWork W2387103395 @default.
- W2377579002 hasRelatedWork W2393375070 @default.
- W2377579002 hasRelatedWork W2393982340 @default.
- W2377579002 hasRelatedWork W2560326540 @default.
- W2377579002 hasRelatedWork W2796409893 @default.
- W2377579002 hasRelatedWork W2797834791 @default.
- W2377579002 hasRelatedWork W2943814737 @default.
- W2377579002 hasRelatedWork W3030067612 @default.
- W2377579002 hasRelatedWork W3141244506 @default.
- W2377579002 isParatext "false" @default.
- W2377579002 isRetracted "false" @default.
- W2377579002 magId "2377579002" @default.
- W2377579002 workType "article" @default.