Matches in SemOpenAlex for { <https://semopenalex.org/work/W2377594333> ?p ?o ?g. }
Showing items 1 to 37 of
37
with 100 items per page.
- W2377594333 abstract "AIM: To examine and compare the ability of serum IgA 1, from both the patients with IgA nephropathy (IgAN) and the healthy control, to bind to human mesangial cells (HMC). METHODS: Serum IgA was isolated with jacalin column, heated to aggregated form (aIgA 1) and labeled with [ 125 I]. Binding capacity of aIgA 1 to primary HMC was evaluated by radioligant binding assay, specificity of binding was determined by competitive inhibition, and relative affinities was compared by cross competitive inhibition. RESULTS: Both aIgA 1 from normal control and patients with IgAN bound to MC in a dose-dependent, saturatable manner, but the binding of aIgA 1 from patients was saturated at approximately 200 pmol while that from healthy was at 400 pmol. The Scatchard analysis revealed a Kd of (8 9±2 1)×10 -8 mol/L for patient's aIgA 1 versus (4 3±1 2)×10 -7 mol/L for normal aIgA 1 ( P 0.05). Competition inhibition showed that both serum albumin and IgG could not block the binding of aIgA 1 to HMC while mIgA 1 could partially block that. Cross competition inhibition demonstrated that patient's aIgA 1 blocked normal aIgA 1 binding significantly ( P 0 01), however, normal aIgA 1 was unable to inhibit the binding of patient's aIgA 1. CONCLUSIONS: ①There are IgA binding proteins or receptors on mesangial cells. ②aIgA 1 from patients with IgAN has a higher binding capacity than that from healthy." @default.
- W2377594333 created "2016-06-24" @default.
- W2377594333 creator A5022728200 @default.
- W2377594333 date "2002-01-01" @default.
- W2377594333 modified "2023-09-25" @default.
- W2377594333 title "Study on the binding capacity of IgA_1 from patients with IgA nephropathy to human mesangial cells" @default.
- W2377594333 hasPublicationYear "2002" @default.
- W2377594333 type Work @default.
- W2377594333 sameAs 2377594333 @default.
- W2377594333 citedByCount "0" @default.
- W2377594333 crossrefType "journal-article" @default.
- W2377594333 hasAuthorship W2377594333A5022728200 @default.
- W2377594333 hasConcept C126322002 @default.
- W2377594333 hasConcept C170493617 @default.
- W2377594333 hasConcept C71924100 @default.
- W2377594333 hasConceptScore W2377594333C126322002 @default.
- W2377594333 hasConceptScore W2377594333C170493617 @default.
- W2377594333 hasConceptScore W2377594333C71924100 @default.
- W2377594333 hasLocation W23775943331 @default.
- W2377594333 hasOpenAccess W2377594333 @default.
- W2377594333 hasPrimaryLocation W23775943331 @default.
- W2377594333 hasRelatedWork W1418428435 @default.
- W2377594333 hasRelatedWork W2039512460 @default.
- W2377594333 hasRelatedWork W2099377647 @default.
- W2377594333 hasRelatedWork W2259934572 @default.
- W2377594333 hasRelatedWork W2318348937 @default.
- W2377594333 hasRelatedWork W2326392414 @default.
- W2377594333 hasRelatedWork W2403303923 @default.
- W2377594333 hasRelatedWork W2424952218 @default.
- W2377594333 hasRelatedWork W2435702787 @default.
- W2377594333 hasRelatedWork W34500584 @default.
- W2377594333 hasRelatedWork W70331615 @default.
- W2377594333 hasRelatedWork W82025115 @default.
- W2377594333 isParatext "false" @default.
- W2377594333 isRetracted "false" @default.
- W2377594333 magId "2377594333" @default.
- W2377594333 workType "article" @default.