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- W2377601622 abstract "The human macrophage colony stimulating factor cDNA(encoding the Nterminal 1-149aa) was inserted into the transfer vector of the BmNPV.After homologous recombination in vivo, the recombinant virus were screened and purified by plaque assay. The cell culture supernatant from the BmN monolayer cells infected with the purified recombinant virus showed the activity of 1.4×105OFU/ml medium on the 6th day after infection. When the silkworm larvae were infected with the recombinant virus, the rhM-CSF was expressed 4-5 days post infection with the activity of 3×106 CFU/ml hemolymph which is equivalent to 300μg M-CSF/ml hemolymph. Western-blotting analysis showed three bands with the molecular weights of 18, 20 and 22kd. The different molecular weights might be attributed by the difference in the degree of glycosylation." @default.
- W2377601622 created "2016-06-24" @default.
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- W2377601622 date "1995-01-01" @default.
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- W2377601622 title "High Level Expression of Human Macrophage Colonystimulating Factor (hM-CSF) in Silkworm" @default.
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