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- W2377643240 abstract "Objective To study the method of isolation and cultivation of neural stem cells from newborn rat spinal cords and identification of its differentiation,to establish cytological foundation for vicariousness therapy of peripheral nerveous system diseases by using spinal stem cells. Methods The neural stem cells were isolated by injector perfusion method from spinal cords of neonatal Wistar rat within 24 h, and induced by serum,the morphological method was used to observe the growth status of cells,anti-Nestin antibody,anti-GFAP antibody and anti-MAP-2 antibody were used for immunocytochemistry test to identify cell differentiation.Results The status of neural stem cells from newborn rat spinal cords was adherence culture in vitro,and the cells could be induced by serum to diffentiate into neural cells with typical morphology and expressing tissue-specific protein Nestin,GFAP,MAP-2. Conclusion The neural stem cells can be isolated from newborn rat spinal cords with adherence proliferation status and they have potential of differentiation." @default.
- W2377643240 created "2016-06-24" @default.
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- W2377643240 date "2009-01-01" @default.
- W2377643240 modified "2023-09-26" @default.
- W2377643240 title "Sepration,cultivation and differentiation of neural stem cells from newborn rat spinal cords" @default.
- W2377643240 hasPublicationYear "2009" @default.
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