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- W2377657215 abstract "A pair of primers were designed based on the 16 S rRNA sequence of Eperythrozoon suis,and a real-time fluorescent quantitative PCR(real-time FQ-PCR) assay was established for the detection of E.suis by using DNA extracted from positive strain and the ferret isolated as templates.Tm value of the specific production was confirmed to be 88.5 ℃.An ordinary PCR was also performed.This technique was highly sensitive with a detection limit of 0.036 fg/μL of positive recombinant plasmid.After the reaction conditions and reaction systems of the real-time FQ-PCR were optimized,a standard curve was obtained with the positive recombinant plasmid diluted by 10 times as template.The developed real-time FQ-PCR using SYBR GreenⅠ was specific,highly sensitive and had broad applications,and could be further used in clinic detection and epidemic investigation of E.suis." @default.
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- W2377657215 date "2007-01-01" @default.
- W2377657215 modified "2023-09-24" @default.
- W2377657215 title "Establishment of a real-time fluorescent quantitative PCR assay for the detection of Eperythrozoon suis" @default.
- W2377657215 hasPublicationYear "2007" @default.
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