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- W2377664054 abstract "Protein identification is a significant bottleneck in proteomic researches of some species with unknown genomes or uncompleted protein database at present. Mass spectrometry driven BLAST (MS BLAST) is a sequence similarity searching tool, which has been developed in recent years and successfully applied to the identification of protein from species with unsequenced genomes. SPITC derivation is an improved de novo sequencing method by Mass Spectrometry. Combining these two methods, 19 proteins of goldfish embryos not identified by Mascot searching were successfully identified by MS BLAST. Among 19 proteins, 12 were identified by MS BLAST searching, while the other 7 were identified by MS BLAST searching based on de novo sequencing of SPITC derivated peptides. This strategy has been proved feasible and reliable for protein identification of goldfish embryos, and provides a new pathway for protein cross-species identification of species with unsequenced genomes." @default.
- W2377664054 created "2016-06-24" @default.
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- W2377664054 date "2006-01-01" @default.
- W2377664054 modified "2023-09-25" @default.
- W2377664054 title "Application of Mass Spectrometry Driven BLAST in Protein Identification of Goldfish Embryos" @default.
- W2377664054 hasPublicationYear "2006" @default.
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