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- W2377668093 abstract "Objective To find a new way to produce S.japonicum (Sj) monoclonal antibodies in large scale. Methods A phage displayed antibody library against Sj was constructed with Sj infected murine spleen. To get the single chain variable fragment antibody (scFv) against Sj, the library was screened with metabolic antigen of the adult worm of Sj (Sj MAg) in ELISA, furthermore, the characteristics of the scFvs were determined. The soluble scFvs selected were used in assaying sera of acute and chronic patients with Schistosomiasis. Results From the phage displayed antibody library with the content about 1.07×106cfu,two specific positive clones with good reactivity were obtained. Specific soluble anti Sj MAg scFvs were produced from these two clones. The results of Western blot showed that the scFvs of both clones could bind to Sj MAg specifically with a molecular weight about 31kDa. Testing the acute and chronic patients with one of scFvs or two scFvs mixed, the sensitivity is 60%, 36.7%and 75%,56.7%respectively and the overall specificity is 85%～90%. Conclusions The results indicated that a phage displayed library against Sj were constructed successfully and some potential scFvs to detect Sj patients had been isolated." @default.
- W2377668093 created "2016-06-24" @default.
- W2377668093 creator A5032723809 @default.
- W2377668093 date "2003-01-01" @default.
- W2377668093 modified "2023-09-25" @default.
- W2377668093 title "Construction and Application of Phage Display Library Against Schistosoma japonicum" @default.
- W2377668093 hasPublicationYear "2003" @default.
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