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- W2377702933 abstract "Reverse transcription PCR(RT-PCR)and rapid amplification of cDNA ends(RACE)technologies were used to clone JrDHN(GenBank accession No. KC503061.1)from leaves of walnut(Juglans regia L.‘Xiangling'). Cloned JrDHN gene was 1 056 bp in length,containing 528 bp ORF encoded 175 amino acids. Amino acid sequence analysis indicated that JrDHN possessed typical characters of LEA family. qRT-PCR analysis showed that the expression of JrDHN in Xiangling walnut was induced by low temperature and peaked at 4 hours after exposed to 4 ℃ low temperature. Under the condition of natural overwintering,JrDHN gene expression increased firstly,reached to a peak in December,followed by decrease. It was deduced that JrDHN may play important roles in tolerating to cold stress. The transcripts of JrDHN could be detected in female flower,bark,flower bud and leaf of Xiangling walnut,especially high expression in female flower. Additionally,after analyzing single nucleotide polymorphism of JrDHN,30 SNPs and 11 Insertions/Deletions(Indels)were detected among 12 walnut accessions. Haplotype analysis showed that 12 walnut accessions were divided into ten haplotypes,and the haplotype diversity was 0.9697." @default.
- W2377702933 created "2016-06-24" @default.
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- W2377702933 date "2014-01-01" @default.
- W2377702933 modified "2023-09-28" @default.
- W2377702933 title "Molecular Cloning, Expression and Single Nucleotide Polymorphisms Analysis of Typical Y_2SK_2 Dehydrin in Juglans" @default.
- W2377702933 hasPublicationYear "2014" @default.
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