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- W2377720675 abstract "Objective To observe the enhancing effect of caffeine on cisplatin in treatment of osteosarcoma cell line OS-732. Methods The osteosarcoma cell line OS-732 was incubated with different concentrations of caffeine,cisplatin and caffeine+cisplatin for 72h.With 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay the cytotoxicity was observed;and the cell cycle phases were analyzed by flow cytometry (FCM). Results The cytotoxicity index (CI) increased as the concentrations of caffeine or cisplatin increased.When the cells were treated with the combination of caffeine and cisplatin,the CI increased more prominently.FCM showed a marked increase of G0 / G1 fraction and decrease of G2 / M fraction of the osteosarcoma cells treated with caffeine. Conclusion Caffeine combined with cisplatin have an enhancing cytotoxicity on osteosarcoma cell line and also can change the cell cycle of the treated osteosarcoma cells." @default.
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- W2377720675 date "2005-01-01" @default.
- W2377720675 modified "2023-09-23" @default.
- W2377720675 title "Caffeine enhances the effect of cisplatin on osteosarcoma cell line OS-732" @default.
- W2377720675 hasPublicationYear "2005" @default.
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