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- W2377748085 abstract "OBJECTIVE:To establish a RP-HPLC precolumn derivatization method for the content determination of argi-nine(Arg)and proline(Pro)in Folium Isatidis.METHOD:2,4-dinitro-fluorobenzene was undergone precolumn derivatization and the amino acids was determined directly under alkaline condition with Kromasil C 18 as chromatographic column,the mobile phase was composed of sodium acetate buffer solution(pH=6.4)-acetonitrile(850∶150)with detection wavelength at360nm,the content was calculated by external reference method.RESULTS:The linear ranges for Arg and Pro were0.627μg～5.016μg(r=0.9996)and0.874μg～7.000μg(r=0.9995),respectively.The average recovery was98.2%with RSD at2.3%and2.2%,respectively.CONCLUSION:The method is simple,accurate and reliable,and suitable for the assaying of amino acids in Folium Isatidis." @default.
- W2377748085 created "2016-06-24" @default.
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- W2377748085 date "2006-01-01" @default.
- W2377748085 modified "2023-09-23" @default.
- W2377748085 title "Content Determination of Amino Acids in Folium Isatidis by RP-HPLC Precolumn Derivatization Method" @default.
- W2377748085 hasPublicationYear "2006" @default.
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