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- W2377775990 abstract "Objective To explore the relationship between G6PD and cancer and its fundamental principles by establishing a sensitive,duplicable and stable system of detecting G6PD mRNA expression.Methods Real-time PCR was used to detect G6PD mRNA expression in the human skin melanoma(A375) cells with wild type,siRNA interference type and siRNA negative sequences type.The standard curves of β-actin and G6PD were drawn,and the sensitivity,stability and repetition of this method were evaluated,and G6PD mRNA expression was corrected by β-actin gene expression.Results The regression coefficients of the standard curves with β-actin and G6PD were 1.086 and 0.940;the coefficient variations(CV) of repetition and stability for Ctβ-actin value were 1.76% and 1.87%,and which were 1.76% and 1.87% for CtG6PD;compared with A375 cells in the wild type,the negative siRNA sequences did not interfere the G6PD mRNA expression(P0.05) and the interference efficiency of the siRNA was 49 %(P0.01).Conclusions Real-time PCR is of high sensitivity,reproducibility and stability,and can be used to study the dependability between the G6PD and cancer." @default.
- W2377775990 created "2016-06-24" @default.
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- W2377775990 date "2007-01-01" @default.
- W2377775990 modified "2023-09-25" @default.
- W2377775990 title "Assay of G6PD Gene Expression in the Human Skin Melanoma by Real-time PCR" @default.
- W2377775990 hasPublicationYear "2007" @default.
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