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- W2377778092 abstract "Objective To evaluate the diagonsis value of M.tuberculosis DNA detected by fluorescence quantitative polymerase chain reaction(FQ-PCR) in sputum and bronchoalveolar lavage fluid(BALF) for patients with smear-negative suspicious pulmonary tuberculosis(PTB).Methods Fifty one suspicious lung tuberculosis patients from July to December in 2009 with at least three times negative sputum smear are divided into two groups: 24 cases with suspicious radiological features of pulmonary tuberculosis(suspicious PTB group) and 27 cases with radiological features of non-tuberculosis(excluding PTB group).All of patients underwent tuberculin skin test,anti-TB antibody and erythrocyte sedimentation rate(ESR) are detected,Mtb-DNA of sputum and BALF are detected qualitatively and quantitatively by FQ-PCR.Results Sputum specimens PCR has a sensitivity of 45.8%(11/24),specificity of 96.3%(26/27),and Youden's index of 42.1%,whereas in BALF specimens,the sensitivity and Youden's index are significantly higher at 75.0%(18/24) and 71.3% respectively,while the specificity is still high at 96.3%(26/27).Conclusion Detecting Mtb-DNA in BALF by FQ-PCR is more sensitive than sputum specimens,and has a good diagnostic yield in patients with suspicious radiological features and sputum smear-negative pulmonary tuberculosis,especially without sputum production." @default.
- W2377778092 created "2016-06-24" @default.
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- W2377778092 date "2011-01-01" @default.
- W2377778092 modified "2023-09-25" @default.
- W2377778092 title "The diagnosis value comparison of Mycobacterium tuberculosis by FQ-PCR in samples for smear-negative pulmonary tuberculosis" @default.
- W2377778092 hasPublicationYear "2011" @default.
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