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- W2377800449 abstract "To explore the mechanism of single use of monoclonal antibody C225 or combined use with DDP,100 μg/ml or 300 μg/ml of monoclonal antibody C225 was singly or in combination with DDP was applied to the cultures of HeLa cells and the inhibitory effect upon the cellular growth was observed 72 hours later.Control without any treatment was also set up.MTT assay was used to detect the inhibitory effect on the growth HeLa cells and the synergistic effect,and flow cytometry technique was applied to find out the influence of 300 μg/ml of C225 singly used or in combined use of various concentrations of DDP upon the cell cycles and apoptosis.Staining technique with HOECHEST33258 was used to detect the morphological changes in cell apopotosis.It was found that monoclonal antibody C225 showed apparently inhibitory effect on the proliferation of HeLa cells and the synergistic or additive effect could be demonstrated in the combined use with DDP.In addition,C225 could also arrest the cell cycle in G1 phase and induce the cell apoptosis,in which the rate of apoptosis was even higher in case of the combined use with DDP and it could further arrest the cell cycle in G2 phase.and reduce the proportion of S-phase.Under fluorescent microscopic examination,the intensity of fluorescence after treatment was weaker than that of the controls,but no apoptotic bodies could be demonstrated.However,certain amount of cells with apoptosis could be found after treatment,and the rate of apoptosis increased with the increased concentration of drug used,in which it appeared to be most prominent in case of the combined use with DDP.From these observation,it suggests that the mechanism of inhibitory effect of monoclonal antibody C225 singly used or in combined use with DDP may be associated to its blocking effect on the cell cycle in G0 to G1 phase and the induction of apoptosis." @default.
- W2377800449 created "2016-06-24" @default.
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- W2377800449 date "2008-01-01" @default.
- W2377800449 modified "2023-09-23" @default.
- W2377800449 title "The mechanism of single use of monoclonal antibody C225 or combined use with DDP upon the in vitro inhibition of HeLa cells" @default.
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