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- W2377801276 abstract "Objective: To establish actinobacillus pleuropneumoniae Type 1 antiserum Actinobacillus actinomycetemcomitans (APP-1) lipopolysaccharide (LPS) monoclonal antibody hybridoma cell lines and to lay a foundation for immunity examination technology for this bacteria. Methods: Intraperitoneal injection of formalin-fixed APP-1 strain vaccine antigen.ELISA antibody titer of serum LPS and choose the mouse with the highest value for cell fusion. Conduct an enhancing immunization three days before Conventional methods of integration. Selectively cultivate fusion cells in HT and HAT medium. Cell culture supernatant is checked for LPS antibody using ELISA. Positive hybridoma cells are cloned using limited dilution. Results: 2 strains secreting anti APP-1 antigen monoclonal antibody hybridoma cell lines of LPS are attained. Mouse ascites monoclonal antibody titer is 1: 32 000 ～ 1:64 000. Conclusion: Three strains secreting anti - LPS APP-1 antigen monoclonal antibody hybridoma cell lines are successfully established, which lay a foundation for future differential diagnosis of colloidal gold technologies for this bacteria. The bacteria build for the future differential diagnosis of colloidal gold technologies." @default.
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- W2377801276 date "2007-01-01" @default.
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- W2377801276 title "Preparation and Identification of the Monoclonal Antibodies of Actinobacillus Pleuropneumoniae in Swine" @default.
- W2377801276 hasPublicationYear "2007" @default.
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