Matches in SemOpenAlex for { <https://semopenalex.org/work/W2377805823> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2377805823 abstract "Objecticve To study the immunogenicity and protective effecacy of MTB8.4 with signal peptide gene vaccine against TB in mice.Methods C57BL/6N!mice were vaccinated with MTB8.4 with signal peptide(MS) gene vaccine three times at 3 weeks intervals.Four weeks after the final vaccination,three mice were sacrificed to assess cytokine response and CTL induction and the other five mice were challenged intravenously in a lateral tail vein with(1×10~(6)) CFU of virulent M.tuberculosis H37Rv.Spleen and the left lung were harvested from each mouse 4 weeks after(infection) and homogenized in sterile saline.Serial dilutions of organ homogenates were plated on (L-J agar) and incubated(37 ℃) until colonies were visible 4 weeks later. Protective efficacy in each experiment was expressed as(reduced) CFU and was compared with the negative control group.The right lung was obtained from each mouse and(inflated) with and stored in 10% formalin saline immediately.Tissues were embedded in paraffin,sec-tioned(and stained) with hematoxylin and eosin.Results MS gene vaccine induced the secretion of more of Th1 cytokines(IFN-γand IL-2),but not IL-4 and enhanced CTL activity while BCG induced the secretion of both types of cytokines(IFN-γ,IL-2 and IL-4).Mice immunized with MS gene vaccine had fewer and smaller tubercles than those immunized with PBS or control plasmid DNA,but had more tubercles than the mice inoculated with BCG.Virable bacterial counts in the lungs and spleens of mice receiving DNA encoding MS were reduced as compared with mice injected with PBS or control plasmid DNA,while increased as compared with mice immunized with BCG.Conclusion MS gene vaccine can induce secretion of Th1 cytokines and the higher protective efficacy against TB in mice." @default.
- W2377805823 created "2016-06-24" @default.
- W2377805823 creator A5010643967 @default.
- W2377805823 creator A5031545203 @default.
- W2377805823 creator A5033022347 @default.
- W2377805823 creator A5033921996 @default.
- W2377805823 creator A5035085609 @default.
- W2377805823 creator A5045132474 @default.
- W2377805823 creator A5079540603 @default.
- W2377805823 date "2006-01-01" @default.
- W2377805823 modified "2023-10-12" @default.
- W2377805823 title "Immuno-protection efficacy of Mtb8.4 gene vaccine with signal peptide against TB in mice" @default.
- W2377805823 hasPublicationYear "2006" @default.
- W2377805823 type Work @default.
- W2377805823 sameAs 2377805823 @default.
- W2377805823 citedByCount "0" @default.
- W2377805823 crossrefType "journal-article" @default.
- W2377805823 hasAuthorship W2377805823A5010643967 @default.
- W2377805823 hasAuthorship W2377805823A5031545203 @default.
- W2377805823 hasAuthorship W2377805823A5033022347 @default.
- W2377805823 hasAuthorship W2377805823A5033921996 @default.
- W2377805823 hasAuthorship W2377805823A5035085609 @default.
- W2377805823 hasAuthorship W2377805823A5045132474 @default.
- W2377805823 hasAuthorship W2377805823A5079540603 @default.
- W2377805823 hasConcept C134018914 @default.
- W2377805823 hasConcept C147969180 @default.
- W2377805823 hasConcept C159047783 @default.
- W2377805823 hasConcept C167508917 @default.
- W2377805823 hasConcept C167672396 @default.
- W2377805823 hasConcept C203014093 @default.
- W2377805823 hasConcept C22070199 @default.
- W2377805823 hasConcept C2777397205 @default.
- W2377805823 hasConcept C2780801004 @default.
- W2377805823 hasConcept C2780868878 @default.
- W2377805823 hasConcept C2780931953 @default.
- W2377805823 hasConcept C71924100 @default.
- W2377805823 hasConcept C86803240 @default.
- W2377805823 hasConcept C8891405 @default.
- W2377805823 hasConcept C89423630 @default.
- W2377805823 hasConceptScore W2377805823C134018914 @default.
- W2377805823 hasConceptScore W2377805823C147969180 @default.
- W2377805823 hasConceptScore W2377805823C159047783 @default.
- W2377805823 hasConceptScore W2377805823C167508917 @default.
- W2377805823 hasConceptScore W2377805823C167672396 @default.
- W2377805823 hasConceptScore W2377805823C203014093 @default.
- W2377805823 hasConceptScore W2377805823C22070199 @default.
- W2377805823 hasConceptScore W2377805823C2777397205 @default.
- W2377805823 hasConceptScore W2377805823C2780801004 @default.
- W2377805823 hasConceptScore W2377805823C2780868878 @default.
- W2377805823 hasConceptScore W2377805823C2780931953 @default.
- W2377805823 hasConceptScore W2377805823C71924100 @default.
- W2377805823 hasConceptScore W2377805823C86803240 @default.
- W2377805823 hasConceptScore W2377805823C8891405 @default.
- W2377805823 hasConceptScore W2377805823C89423630 @default.
- W2377805823 hasLocation W23778058231 @default.
- W2377805823 hasOpenAccess W2377805823 @default.
- W2377805823 hasPrimaryLocation W23778058231 @default.
- W2377805823 hasRelatedWork W1978957008 @default.
- W2377805823 hasRelatedWork W2137129186 @default.
- W2377805823 hasRelatedWork W2348019389 @default.
- W2377805823 hasRelatedWork W2348020497 @default.
- W2377805823 hasRelatedWork W2349745957 @default.
- W2377805823 hasRelatedWork W2351677845 @default.
- W2377805823 hasRelatedWork W2352190125 @default.
- W2377805823 hasRelatedWork W2360900373 @default.
- W2377805823 hasRelatedWork W2362225300 @default.
- W2377805823 hasRelatedWork W2363322663 @default.
- W2377805823 hasRelatedWork W2365343937 @default.
- W2377805823 hasRelatedWork W2368753468 @default.
- W2377805823 hasRelatedWork W2372570488 @default.
- W2377805823 hasRelatedWork W2374302986 @default.
- W2377805823 hasRelatedWork W2379167136 @default.
- W2377805823 hasRelatedWork W2380570081 @default.
- W2377805823 hasRelatedWork W2385718441 @default.
- W2377805823 hasRelatedWork W2385964128 @default.
- W2377805823 hasRelatedWork W2394183548 @default.
- W2377805823 hasRelatedWork W3032253552 @default.
- W2377805823 isParatext "false" @default.
- W2377805823 isRetracted "false" @default.
- W2377805823 magId "2377805823" @default.
- W2377805823 workType "article" @default.