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- W2377807061 abstract "Objective To develop new reagents for future studies on the role of CTLs in protection against rotavirus infection and vaccine design/evaluation in humans,HLA-A*0201 restricted CTL epitopes from protein VP6 of rotavirus Wa strain were predicted and identified.Methods A computer-based program,which is accessed through SYFPEITHI combined with PAProc/Fragpredict proteasomal cleavage site prediction algorithm and molecular modeling was utilized to predict HLA-A*0201 restricted CTL epitopes within rotavirus protein VP6.Candidate peptides were synthesized and purified by reverse phase HPLC,and analysed by mass spectrometry.T2 cells were used to determine the peptide binding affinity and HLA-A*0201-peptide complex stability.Results Six peptides with the highest score predicted by SYFPEITHI and proteasomal cleavage site at the C-terminal were chosen and then two of them were eliminated through molecular modeling.Finally,four candidate peptides were synthesized and purified to over 95% by reverse phase HPLC and confirmed by mass spectrometry.Among the four predicted peptides,TLLANVTAV(340-348)and VLADANETL(333-341)could bind to HLA-A*0201 with high affinity,the value of flurorescene index(FI) was 2.66 and 2.64 respectively,and the dissociation time of 50% HLA-A*0201-peptide complex was over to 8 h.Conclusion Our results suggest that TLLANVTAV(340-348)and VLADANETL(333-341)might be HLA-A*0201 restricted CTL epitopes." @default.
- W2377807061 created "2016-06-24" @default.
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- W2377807061 date "2006-01-01" @default.
- W2377807061 modified "2023-09-26" @default.
- W2377807061 title "Primary identification of HLA-A*0201-restricted CTL epitopes from rotavirus protein VP6" @default.
- W2377807061 hasPublicationYear "2006" @default.
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