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- W2377842513 abstract "Objective:To investigate the effects of arsenic trioxide(ATO) on the levels of IFN-γexpression and its mRNA transcripts in splenic CD4+T cells from MRL/lpr mice.Methods:CD4+T cells were isolated from the spleens of MRL/lpr and C57BL/6J mice as control via magnetic bead separation at 18 to 20 weeks of age.Cell purity was checked by flow cytometry.CD4+ T cells were stimulated for 48 h in the presence of PHA-p(20μg/ml)and IL-2(1 000 IU/ml).Then cells were treated with ATO(0.5,1.0 and 2.0μmol/L) for 24 h,which were also exposed to 1.0μmol/L ATO treatment for 12,24 and 48 h.In addition,cells were added with ATO,or 5-azacytidine(5-AzaC) or ATO combined with 5-AzaC at 1.0μmol/L,respectively,as compared to those given PBS.Enzyme linked immunosorbent assay(ELISA)was used to measure serum levels of IFN-γ.A real-time fluorescent quantitative PCR(FQ-PCR) was set up to determine IFN-γ mRNA expression in different drugs-treated-CD4+T cells.Results:①ATO(1.0μmol/L) was of no effect on survival rate of cells cultured for 24 h.②After treated with 1.0μmol/L ATO for 24 h,transcription and interpretation levels of IFN-γin splenic CD4+T cells from MRL/lpr mice were inhibited.③In the presence of 5AazC,the levels mentioned above markedly increased in MRL/lpr and C57BL/6J mice,which could be also inhibited by 1.0μmol/L ATO after a 24-h incubation.④The production and mRNA expression of IFN-γ of splenic CD4+T cells were significantly higher in MRL/lpr mice,compared to C57BL/6J mice.Conclusion:The treatment of splenic CD4+T cells with 1.0 μmol/L ATO by culturing for 24 h could efficiently inhibit abnormal secretion level of IFN-γ in MRL/lpr mice,and down-regulate mRNA level without affecting the survival rate." @default.
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- W2377842513 date "2009-01-01" @default.
- W2377842513 modified "2023-09-27" @default.
- W2377842513 title "Effects of arsenic trioxide on the levels of IFN-γ expression and mRNA transcripts in splenic CD4+ T cells from MRL/lpr mice" @default.
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