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- W2377852785 abstract "Meningitis is one of the main human acute respiratory diseases, on the purpose of development polysaccharide vaccine of the A, C, W135, and Y, we investigated the different culture conditions of meningococcal group W135 with the 50 liters fermentor. The observation was carried out on the influence of the factors, such as glucose concentration, culture temperature, pH, aerating quantity and mixing rate on the growth of W135, so as to select the optimal culture condition. The semi-synthesis medium mended with 1% glucose showed good effect and the seed concentration had a direct effect on the polysaccharide yield. The W135 group was able to maintain high speed growth in the optimal pH range 6.5~7.5 and obtained high cultural products at 36.5±0.2℃. Enlargement of the aerating quantity and the mixing rate can apparently improve the culture results during the culture process. The optimal culture condition of group W135 was determined in this study. After three batches of consecutive culture in the 500 liters fermentor, the results achieved the standards on large scale for the manufacture requirement." @default.
- W2377852785 created "2016-06-24" @default.
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- W2377852785 date "2005-01-01" @default.
- W2377852785 modified "2023-09-25" @default.
- W2377852785 title "Study on ferment culture of meningococcus group W135" @default.
- W2377852785 hasPublicationYear "2005" @default.
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