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- W2377854513 abstract "[Objective]To screen proteins binding with α-defensin(HNP-1)mature peptide from placenta cDNA libraty by yeast two hybrid technique.[Methods]The cDNA fragment encoding HNP-1 mature peptide was amplified by polymera-sechain reaction(PCR)and constructed into pGBK-T7 vector as the bait plasmid in yeast two hybrid system 3.Subsequently,the RNA from fetal adrenal gland was obtained and then transformed into cDNA library using SMART technology.The fetal adrenal cDNA library was screened with pGBKT7-HNP-1 as bait plasmid by yeast-two hybrid system Matchmaker Lexa.Finally,the positive clone was obtained by PCR and then identified by sequence.Then the interaction between them was determined by GST pull down in vitro and coimmunoprecipitates experiments in vivo.[Results]Bait and cDNA library have been constructed successfully and transformed into yeast.Then the interaction protein was found--melanocortin 2 receptor(ACTH-R),CCAAT-enhancer-binding proteins(C/EBP),Tramembrane trafficking protein(TMP21),low density lipoprotein receptor-related protein 6(LRP6).Therefore,melanocortin 2 receptor(ACTH-R)was determined into the major subjects.And bands which can demonstrate the relationship between HNP-1 and ACTH-R was obtained in GST pull down and coimmunoprecipitates experiments.[Conclusion]ACTH-R can bind to HNP-1 we obtained from fetal adrenal cDNA library and it may play important roles in the function of HNP-1 mature peptide." @default.
- W2377854513 created "2016-06-24" @default.
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- W2377854513 date "2011-01-01" @default.
- W2377854513 modified "2023-09-24" @default.
- W2377854513 title "SCREENING THE GENE SEQUENCES OF THE INTERACTION PROTEINS OF HNP-1 FROM HUMAN FETAL ADRENAL CDNA LIBRARY BY YEAST TWO HYBRID SYSTEM" @default.
- W2377854513 hasPublicationYear "2011" @default.
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