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- W2377857044 abstract "Objective To investigate the change of caspase-3 and F-actin in the apoptosis of prostate cancer androgen-independent cell line DU145 induced by all-trans retinoic acid (ATRA). Methods Using AO dying and fluorescent microscope to observe the shape of the cell,and the apoptosis peak can be observed by FACScan too.The effects of ATRA on the expression of caspase-3 was analyzed by Western blot and the F-actin’s structure was observed by immunofluorescent microscope. Results Flurescent microscope can observe more apoptotic cells about 85.0% in 48 h and FACScan can observe obvious apoptotic peak come into being after treated by ATRA. Meanwhile, the expression levels of cleaved caspase-3 was up-regulated.The structure of F-actin was destroyed. Conclusions ATRA could induce the apoptosis of DU145 cell mediated by caspase-3 and lead to the destroy of F-actin’s structure." @default.
- W2377857044 created "2016-06-24" @default.
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- W2377857044 date "2006-01-01" @default.
- W2377857044 modified "2023-09-23" @default.
- W2377857044 title "The Change of caspase-3 and F-actin in the apotosis of prostate cancer androgen-independent cell line DU145 induced by ATRA" @default.
- W2377857044 hasPublicationYear "2006" @default.
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