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- W2377860128 abstract "Objective To investigate the curcumin-induced demethylation of the promoter of peroxisome proliferator-activated receptor α(PPAR-α) in the hepatocyte steatosis models induced by oleic acid.Methods The normal rat hepatocytes(BRL) were divided into 4 groups:normal group(DMSO for 96 hours),non-alcoholic fatty liver disease(NAFLD) group,curcumin group,and 5-Aza-CdR group.In NAFLD,curcumin and 5-Aza-CdR groups,the cells were cultivated with oleic acid(15 μg/mL) for 48 hours,and subsequently oleic acid alone,plus curcumin(10.0 μmol/L),or plus 5-Aza-CdR(5.0 μmol/L) were given to the subjects in NAFLD,curcumin or 5-Aza-CdR group,respectively,for the next 48 hours.Biochemical assays were used to assess the ALT,AST,TG,TC levels in supernatant and TG,TC contents in cells.Oil red O staining was used to observe the fatty droplets in cells.The mRNA expression level of PPAR-α was measured by real time reverse transcription-polymerase chain reaction.The DNA methylation level was analyzed by pyro-sequencing.Results Compared with the normal group,significant increase was observed in supernatant ALT,TG,TC levels and cellular TG,TC contents in the NAFLD group(P 0.05,P 0.01),so was the down-regulation of PPAR-α mRNA(P 0.01).However,the DNA methylation of CpG-378,-375,-373 was significant higher in NAFLD group(P 0.05,P 0.01).Compared with NAFLD group,significant reduction in the supernatant ALT,TG,TC levels and cellular TG,TC contents were observed in curcumin group;so was the reduction of cellular fatty droplets through Oil red O staining.Besides,the expression of PPAR-α mRNA in curcumin group was significantly up-regulated than that of NAFLD group(P 0.01).In addition,the DNA methylation of CpG-378,-375,-373 in curcumin group was significantly lower than that of NAFLD group(P 0.05,P 0.01).Compared with NAFLD group,the expression of PPAR-α mRNA of 5-Aza-CdR group was significantly up-regulated(P 0.01),though it was still lower than that of normal group;and the DNA methylation of CpG-378,-375,-373 were also significantly reduced(P 0.05,P 0.01),however,there was no significant difference between 5-Aza-CdR group and normal group.A significantly negative correlation was revealed between the PPAR-α mRNA expression and DNA methylation of CpG-378(r =-0.663,P = 0.026).Conclusion Curcumin increases the expression of PPAR-α mRNA through the demethylation on CpG promoters,thereby ameliorating NAFLD hepatocyte steatosis." @default.
- W2377860128 created "2016-06-24" @default.
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- W2377860128 date "2014-01-01" @default.
- W2377860128 modified "2023-09-22" @default.
- W2377860128 title "The effects of curcumin on the demethylation of peroxisome proliferator-activated receptor-α of steatosis cells" @default.
- W2377860128 hasPublicationYear "2014" @default.
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