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- W2377877240 abstract "Objective To prepare pneumococcal conjugate vaccine using pneumolysin as a protein carri- er and evaluate its immunogenicity in mice.Methods The DNA fragment encoding for pneumolysin was amplified by PCR and cloned into plasmid pET-28a,and then the recombinant DNA was transfered to host cell E.coli JM109(DE3).The recombinant pneumolysin(rPn)was expressed in vivo and purified by Ni-NTA.The SDS- PAGE was used to identify the rPn.This protein was detoxified by 0.2% or 0.5% formalin.Pneumococcal po- lysaccharide(PS,19F serotype)was conjugated with detoxified rPn using amino reductive method.The conjugate was mixed with the adjuvant Al_2(OH)_3 before vaccination.The antibody response against PS or Pn was measured by ELISA.Results The inserted pneumolysin gene sequence was confirmed by DNA sequencing and the pneumo- lysin protein was successfully expressed.The relative molecular mass of the expressed product was 52×10~3.This recombinant pneumolysin was detoxified by 0.2% or 0.5% formalin.The PS(19F serotype)was successfully conjugated with rPn.There was a significant difference of antibody titer against PS or Pn between vaccinated and non-vaccinated mice(P＜0.01).Conclusion The recombinant pneumolysin can obtained by genetic engineering technology.The PS and rPn conjugate vaccine produced by amino reductive method induced specific immune re- sponse in mice." @default.
- W2377877240 created "2016-06-24" @default.
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- W2377877240 date "2006-01-01" @default.
- W2377877240 modified "2023-09-26" @default.
- W2377877240 title "The experimental research of tne preparation for pneumococcal conjugate vaccine using pneumolysin as a protein carrier and its immumogenicity in mice" @default.
- W2377877240 hasPublicationYear "2006" @default.
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