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- W2377885730 abstract "AIM:To construct galU-deletion mutant of streptococcus pneumoniae and get the capsule deficient strain, which can be used in pneumococcal functional genome research. METHODS: LFH-PCR was introduced to generate a gene disruption construct consisting of Emr cassette with long flanking homology regions to the target gene. The streptococcus pneumoniae was then transformed directly with this PCR product. The galU-deletion mutant was obtained on the TSA agar containing erythromycin and identified by PCR. The deficient strain (5×10 6 CFU) was applied to infect BALB/c mice to determine the virulence. RESULTS: GalU gene was replaced completely by erm cassette. The mutant had low virulence to BALB/c mice. CONCLUSION: The galU-deletion mutant can be used to screen in vivo-promoter in streptococcus pneumoniae because it is incapable of synthesizing capsular polysaccharide and hence loses virulence. The target gene can be replaced completely by LFH-PCR product, so this method is simple, rapid and effective for functional genome research of streptococcus pneumoniae." @default.
- W2377885730 created "2016-06-24" @default.
- W2377885730 creator A5023846029 @default.
- W2377885730 date "2004-01-01" @default.
- W2377885730 modified "2023-09-28" @default.
- W2377885730 title "Construction and function study of galU-deletion mutant in streptococcus pneumoniae" @default.
- W2377885730 hasPublicationYear "2004" @default.
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