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- W2377888851 abstract "Establishment of a nested RT-PCR method for detection CSFV,the purpose of the sequenced fragments identified pandemic or vaccine CSFV.According to published on the GenBank CSFV gene sequence,designed and synthesized two pairs of primers,established a test method of CSFV by nested RT-PCR,amplification of vaccine,SXYL and SX01 strains fragment,analysis these sequences.The result of sensitivity showed that the most dilution cDNA of CSFV is 3.4×10-5 ng/mL;the result specialization showed that the method could not acquire any band from PRRSV,SIV,PCV2 and PRV.Sequence analysis showed that only vaccine strain 3′-NCR has molecular markers CTTTTTTCTTTT,SXYL and SX01 strains had not it.The nested RT-PCR method of detection CSFV was high sensitivity and specificity,it was a viable method of detection.Through fragments of sequence analysis,looking for molecular markers CTTTTTTCTTTT,could accurately identify the pandemic or vaccine CSFV strains." @default.
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- W2377888851 date "2009-01-01" @default.
- W2377888851 modified "2023-09-23" @default.
- W2377888851 title "Identify the Pandemic or Vaccine CSFV Strains by Molecular Markers" @default.
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