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- W2377914234 abstract "Background and purpose:Prostate cancer is one of the most common malignant tumors with a high morbidity and mortality in the urologic diseases, and new approaches have been under intensive investigation for the improvement of clinical outcome. IL-12 plays an important role in anti-tumor immunity and in promotion of proliferation, differentiation of T cell.DC vaccine loaded with tumor antigens can stimulate human body to produce specific immunological anti-tumor effect.This study is to investigate the efficacy and mechanisms of specific anti-tumor immunity induced by using retrovirus-mediated murine interleukin-12 gene-modified dendritic cells sensitized by HSP70-PC-3m peptide against PC-3m cells in vitro. Methods:To construct retrovirus vector targeting mIL-12 gene and to collect virus from PT67.DCs, cells were isolated from the peripheral blood of PCa patient and were transfected by retrovirus-IL-12.IL-12 and IFN-r levels in culture supernatant of DC-IL-12 were measured by ELISA. The capacities of HSP70-PC-3m peptide-pulsed DC-IL-12 in terms of induction of proliferation and differentiation of T lymphocytes were evaluated by MTT.The cytotoxicity of CTL induced by both DC-IL-12 and its culture supernatant against PC-3m and EJ were assessed.Results:48h after IL-12 gene transfection,DC-IL-12 expressed higher levels of IL-12 (26.35±3.12)ng/L and IFN-r(778±28)ng/L as compared with DCs (P0.05).CTL induced by DC-IL-12 and its culture supernatant against PC-3m has shown more potent cytotoxicity in comparison with DCs, cell death were induced 76.43±6.20% by DC-IL-12 vs. 42.12±2.80% by DCs, and (66.38±7.56% by DC-IL-12's culture supernatant vs. 14.23±5.42% by DC's culture supernatant (P0.05). CTL induced by DC-IL-12 and its culture supernatant had no obvious killing effect to EJ.Conclusions:IL-12 modification enhanced the cytotoxic capacity of DC sensitized by HSP70-PC-3m peptide so that it induced autologous T cell to develop specific anti-tumor immunity against PC-3m. The mechanisms might be related to the increased expression of IL-12 、IFN-r and enhanced induction of CTL." @default.
- W2377914234 created "2016-06-24" @default.
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- W2377914234 date "2006-01-01" @default.
- W2377914234 modified "2023-09-26" @default.
- W2377914234 title "In vitro study on specific anti-tumor immunity induced by using retrovirus-mediated murine interleukin-12 gene-modified dendritic cells sensitized by HSP70-PC-3m peptide against prostate carcinoma" @default.
- W2377914234 hasPublicationYear "2006" @default.
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