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- W2377922218 abstract "Objective To investigate the role of tissue factor (TF) in the in vivo hematogenous metastatic ability of human colorectal carcinoma cells (LoVo). Methods The eukaryotic expression vectors pcDNA3.1/Zeo bearing either sense or antisense TFcDNA were transfected into LoVo cells by the way of lipofactamine 2000. TF proteins in transfected cells were detected by Western blot. Twenty-four nude mice were randomized into three groups. The transfected and untransfected LoVo cells were inoculated into the spleens of 24 nude mice (BALB/C Nu/Nu) to produce diffuse liver metastasis respectively. Six weeks later the nude mice were sacrificed, and the number of metastasic nodules on the liver surface was counted to show the metastatic ability of LoVo cells. Results LoVo cells with sense-TFcDNA transfection showed an increased TF expression and the number of metastasic nodules on the liver surface as compared with the cells without transfection, but LoVo cells with antisense-TFcDNA transfection got the contrary change. Conclusion TF couldincrease the hematogenous metastatic ability of human colorectal carcinoma cells (LoVo) in vivo." @default.
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- W2377922218 date "2006-01-01" @default.
- W2377922218 modified "2023-09-22" @default.
- W2377922218 title "The role of tissue factor in the liver metastasis of colorectal carcinoma cells in vivo" @default.
- W2377922218 hasPublicationYear "2006" @default.
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