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- W2377928318 abstract "Objective To express recombinant human mannan-binding lectin N-terminal deletant(rhMBL△N)protein possessing biological activities in E.coli.Methods The rhMBL△N gene was amplified by PCR from pGEM-MBL plasmid,and then inserted into prokaryotic expression vector pET43.1a.After identification by restriction mapping and sequencing,the recombinant plasmid pET43.1a/His rhMBL△N was transformed into E.coli BL21(DE3)cells.The expressed product was purified by immobilized metal affinity chromatography(IMAC),and then identified by SDS-PAGE,Western blotting,and ELISA.Results The cDNA fragment of about 620 bp was amplified from pGEM-MBL plasmid and the recombinant expression vector pET43.1a/His rhMBL△N was constructed,whose restriction maps and sequence were consistent with those expected.A components of Mr 97 000 in the purified recombinant product was detected by SDS-PAGE and could be recognized by anti-6His antibody.The purified recombinant product could react with the antibody against the recombinant human MBL protein and could bind to mannan and MBL associated serine protease 1/2-N proteins specifically.Conclusion The prokaryotic expression strains that express efficiently rhMBL△N and the rhMBL△N protein with biological activities are obtained successfully,which will help the further research of MBL molecule." @default.
- W2377928318 created "2016-06-24" @default.
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- W2377928318 date "2008-01-01" @default.
- W2377928318 modified "2023-09-25" @default.
- W2377928318 title "Prokaryotic expression and biological activities of recombinant human MBL N-terminal deletant(rhMBL△N)" @default.
- W2377928318 hasPublicationYear "2008" @default.
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