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- W2377962570 abstract "This paper describes briefly a new concept for enhancing the resolution of microscope and gives the experimental results for low power and medium power objectives.Two symmetrically oblique coherent light beams are used to illuminate an object and produce two superimposed diffracted spatial frequency spectra at the back focal plane of the objective.One spectrum contains successive diffraction orders 0, 1, 2, …, 2N, the other spectrum contains -2N, …, -2, -1, 0. The greater the numerical aperture of microscope objective we use, the more diffraction orders will be contained in each spectrum and hence the better resolution will be obtained. By using two oblique coherent light beam illumination, the diffraction orders are increased twice as in the single light beam illumination. At first glance, it seems that the resolving power of the objective should increase twice in correspondence with diffraction orders. But this isn't the case, because these two spectra are overlapped at the back focal plane of the objective. In order to attain high resolution, we use a scheme shown in fig. 4 for separating the two spectra and properly recombining them before the diffraction orders as-2N, -2N +1,…, -1, 0,1, - , 2N-1, 2N, instead of superposition-2N, -2N + 1, …, -1, 00, 1, …, 2N-1, 2N.In experiments we observed specimens such as grating, lancet or diatom with objectives 8×(N. A. 0.24) or 20×(N. A. 0.45). By analyzing the microscopic picture taken at different conditions, we conclude that the frequency-combined microscope is superior to the conventional one in resolving power, whether using oblique illumination or not, and it can reveal finer textures of such specimens as lancet and diatom mentioned above and achieve a high contrast in intensity in the case of a grating." @default.
- W2377962570 created "2016-06-24" @default.
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- W2377962570 date "1981-01-01" @default.
- W2377962570 modified "2023-09-25" @default.
- W2377962570 title "A frequency-combined microscope" @default.
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