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- W2377984755 abstract "Objective To construct replication-deficient recombinant adenoviruses containing HIV-1 tat gene and express Tat protein in various cells for establishing a foundation of DC vaccine.Methods HIV-1 tat gene fragment was obtained by PCR and cloned into shuttle vector pTrack-CMV,and then transformed into E.coli BJ5183 containing adenoviruses backbone pAd-easy-1 after linearization at a Pme Ⅰ site.The resulting recombinant plasmids,designated prAd-tat,were digested by Pac Ⅰ and then transfected into packaging cell lines 293 cells with Lipofectamin 2000 to get infective replication-deficient recombinant adenoviruses vAd-tat.Results HIV-1 tat gene was correctly cloned into adenoviruses vector,which was identified by digestion and sequencing;tat could be found in the supernatants by using PCR,and its expression was detected in 293 cells and Hela cells by Western blotting.Conclusion The replication-deficient recombinant adenoviruses is constructed and the tat protein is expressed in 293 cells and Hela cells successfully." @default.
- W2377984755 created "2016-06-24" @default.
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- W2377984755 date "2009-01-01" @default.
- W2377984755 modified "2023-09-26" @default.
- W2377984755 title "Construction of replication-deficient recombinant adenoviruses containing HIV-1 tat gene and its expression in 293 cells" @default.
- W2377984755 hasPublicationYear "2009" @default.
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