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- W2377984859 abstract "Objective To isolate and purify spermatogonia from 2 month|old Changbai boar testis. Methods After enzymatic digestions of boar testis, the suspension passed the 2%|4% BSA continuous gradient medium in a gravity unit by velocity sedimentation. Then the spermatogonia were further purified by attachment culture. Results The purity of spermatogonia was 91% after velocity sedimentation separation.94% of spermatogonia was obtained after further pruification by attachment culture.Conclusion The method of isolating spermatogonia is convenient, fast and efficient. It could meet the needs for molecular studies on spermatogonia. [" @default.
- W2377984859 created "2016-06-24" @default.
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- W2377984859 date "2002-01-01" @default.
- W2377984859 modified "2023-09-26" @default.
- W2377984859 title "SEPARATION AND PURIFICATION OF SPERMATOGONIA FROM CHANG-BAI BOAR TESTIS" @default.
- W2377984859 hasPublicationYear "2002" @default.
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