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- W2378045672 abstract "ObjectiveTo construet recombinant adenoviral vector containing Cox-2L promoter and SSTR2 gene so as to observe the effects of SSTR2 re-expression and adenovirus infection on the growth of pancreatic cancer cells.MethodsHuman SSTR2 eDNA was amplified by RT-PCR from nor- mal human pancreas tissue;Cox-2L DNA was amplified by PCR from human HL 60 cells.Cox-2L-SSTR2 fusion gene was amplified by PCR and the pAxewit Adenoviral Vector System was used to generate repli- cation defective adenoviral vector carrying Cox-2L-SSTR2 fusion gene.The recombinant adenoviral vec- tors were confirmed by PCR and restriction enzyme digestion analysis.The pAxewit Cox-2L-SSTR2 was transfected into HEK293 cell through lipofeetamine and identified by PCR and restriction enzyme diges- tion analysis.ResultsThe three fragments were confirmed to be Cox-2L gene,SSTR2 gene and Cox-2L- SSTR2 fusion gene by sequence analysis.The gene fragments of Cox-2L-SSTR2 and PoiyA were cloned into PMD18-T vector to construct PMD18-T-Cox-2L-SSTR2-PolyA and then confirmed by restriction enzyme digestion and sequence analysis.The constructed recombinant adenoviral vector was detected by PCR and restriction enzyme digestion to confirm that Cox-2L-SSTR2-PolyA fusion gene was correctly cloned into adenoviral vector.ConclusionThe recombinant adenoviral vector containing Cox-2L and SSTR2 genes was eonatrueted successfully.This results lays the foundation for further study on function of SSTR2 in pancreatic cancer cell." @default.
- W2378045672 created "2016-06-24" @default.
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- W2378045672 date "2006-01-01" @default.
- W2378045672 modified "2023-09-23" @default.
- W2378045672 title "Construction of gene vector containing somatostatin receptor type II gene and function of cyclooxygemine-2 promoter" @default.
- W2378045672 hasPublicationYear "2006" @default.
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