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- W2378054013 abstract "OBJECTIVE:To establish a method for the content determination of related substances in Triamcinolone acetonide and econazole nitrate cream. METHODS:HPLC method was adopted. The determination was performed on Venusil ASB C18 column with mobile phase A composed of methanol-0.077% ammonium acetate(20∶80,V/V,pH value adjusted to 4.5 using phosphoric acid)and methanol-acetonitrile(40∶60,V/V)as mobile phase B(gradient elution)at the flow rate of 1.0 ml/min. The detection wavelength was 225 nm,and the column temperature was 35 Ⅴ. The sample size was 10 μl. RESULTS:Fluoxyprednisolone and miconazole nitrate and unknown impurity were completely separated from main components of Triamcinolone acetonide and econazole nitrate cream. The linear range of 2 kinds of known impurities were 1.071-8.568 μg/ml(r=0.999 8)and 1.039-8.312 μg/ml(r=0.999 6). The detection limits were 5.15 ng and 7.31 ng,and quantitative limits were 15.69 ng and 21.97 ng. CONCLUSIONS:The method is sensitive,accurate specific and reproducible,which can be used for the quality control of related substance in Triamcinolone acetonide and econazole nitrate cream." @default.
- W2378054013 created "2016-06-24" @default.
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- W2378054013 date "2014-01-01" @default.
- W2378054013 modified "2023-09-23" @default.
- W2378054013 title "Determination of Related Substance in Triamcinolone Acetonide and Econazole Nitrate Cream by HPLC" @default.
- W2378054013 hasPublicationYear "2014" @default.
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