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- W2378065912 abstract "Objective To treat pancreatic injury of model rats induced by bone marrow mesenchymal stem cells(BM-MSCs)in vitro,and explore methods promoting repair of pancreatic injury.MethodsBM-MSCs were segregated and purified by adherence method to in rats,then used to treat pancreatic injury of model rats after induced by basic fibroblast growth factor(bFGF),epidermal growth factor(EGF),hepatocyte growth factor(HGF),2%B27,active A,β-mercaptoethanol and nicotinamide 12[14 days,identified by RT-PCR,marked by DAPI.One week later,the migrations and locations of islet-like cells were observed by Laser Scanning Confocal Microscope(LSCM),and the source of cells participating in reparation of pancreatic injury was identified by RT-PCR.ResultsAfter 12～14 days,BM-MSCs were induced into the islet-like cells expressing Ins1 and Ins2.The islet-like cells were used to treat pancreatic injury after marked DAPI.One week later,the cells were discovered in injured pancreatic tissues,where inflammatory cells infiltration obviously,cell structure confusion,and pancreatic islet disintegration,and the injured pancreatic tissues began to recover,pancreatic islet re-established.The result of RT-PCR further confirmed that there were infused islet-like cells in injured pancreatic tissues.ConclusionsBM-MSCs can be induced into islet-like cells,which participate in reparation of pancreatic injury." @default.
- W2378065912 created "2016-06-24" @default.
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- W2378065912 date "2009-01-01" @default.
- W2378065912 modified "2023-09-25" @default.
- W2378065912 title "Therapeutic effect of islet-like cells induced by bone marrow mesenchymal stem cells BM-MSCs on pancreatic injury of rat" @default.
- W2378065912 hasPublicationYear "2009" @default.
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