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- W2378085282 abstract "Whether the exo-chitinase gene can be stably inherited in the transgenic tobacco was studied. Firstly, using MS medium containing Kanamycin (100mg/L) geminated the T_2 ,T_3 and T_4 generation transgenic tobacco seeds to test whether the transgenic tobacco seeds contain the NPT II gene, which is a selectable marker gene. Secondly analyzed the protein of transgenic tobacco leaves by Western Blot to test whether the exo-chitinase gene still express protein in the T_2 and T_3 generations. In the Km resistance detection experiment, 11 T_2 transgenic lines were tested. Among them 9 lines 100% seeds have resistance to Km. 10 T_3 and 10 T_4 transgenic lines were tested; all tested seeds have resistance to Km. In the western blot experiment, 11 T_2 transgenic tobacco lines were tested. And the experimental results showed that all tested plants in 6 lines contain the chitinase protein; 80% tested plants of 2 lines contains exo-chitinase protein; 50% tested plants of 2 lines contains exo-chitinase protein; and in 1 line 19.05% tested plants contain exo-chitinase protein. Meanwhile whether the transgene can cause gene flow through wind pollination was also studied. In the green house under the mimic natural wind condition, both transgenic and untransgenic tobacco plants are planted together for two generations. The seeds of each plant were collected separately, and geminated in the MS medium-containing Km (100mg/L). No untransgenic tobacco seeds have resistance to Km were found." @default.
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- W2378085282 date "2005-04-30" @default.
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- W2378085282 title "Study on the inheritance stability of chitinase expressing transgenic tobacco" @default.
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