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- W2378131368 abstract "Objective To appraise the feasibility of transfecting hypoxia inducible factor(HIF)-1αgene into EPCs and to study the influence on the phenotypes. Methods pEGFP-HIF-1αwas successfully constructed with molecular biochemistry. After mononuclear cells were isolated from the human peripheral blood, they were cultured in conditional medium or sorted by MACS(magnetic bead separation method) CD34+ for further culture. Then EPCs were transfected by HIF/1αof pEGFP. Transfection efficiency was detected, and gene expression tested by RT-PCR. The cells were also subjected to FACS to examine the surface expression of CD31. Results After the construction of pEGFP-HIF-1α. The nucleotide sequence insertion was the same as the sequence of HIF-la in Genebank. The cells were transfected and observed 24 hours afterwards. In PBMC, MNC CD34+ , the transfection efficiency was up to 38% , 42% , respectively. Gene expression of HIF-1αwas also tested by RT-PCR. Either cytokine stimulated group or transfected group attained similar expression rate of EC(endothelial cell) marker, greater than the untreated cells. No significant statistical differences were observed in MNC CD34+ groups. Conclusion HIF-1αgene transfection is as effective as the cytokine in promoting the differentiation into ECs in vitro which provides a promising method for amplification of EPCs. (Shanghai Med J, 2006, 29:737-740)" @default.
- W2378131368 created "2016-06-24" @default.
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- W2378131368 date "2006-01-01" @default.
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- W2378131368 title "Study on relationsbip of bypoxia inducible factor-1αand progenitor endothelial cells differentiation in vitro" @default.
- W2378131368 hasPublicationYear "2006" @default.
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