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- W2378264476 abstract "The internal transcribed spacer(ITS)of DNA nucleotide fragments of Trichuris sp.isolated from Black Goat in Hainan region were ampliy by PCR of conserved primers NC5 and NC2.The PCR fragments were purified and cloned into pEASY-T1 vector.The inserts were successfully sequenced,and the results revealed ITS inserts were 1 113 bp in length and consisted of partial 18S,28S and complete ITS1(470 bp),5.8S(158 bp) and ITS2(392) rDNA sequences.ITS1 and ITS2 sequences shared 90%,99% homology with T.skrjabini(Baskakov),and T.leporis(Froelich) is 84%,and lower homology with other nematodes.The results of this study laid a foundation for further studies." @default.
- W2378264476 created "2016-06-24" @default.
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- W2378264476 date "2010-01-01" @default.
- W2378264476 modified "2023-09-23" @default.
- W2378264476 title "Cloning and Sequence Analysis of the rDNA-ITS of Trichuris sp. in Black Goat in Hainan" @default.
- W2378264476 hasPublicationYear "2010" @default.
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