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- W2378269469 abstract "Chitooligosaccharide(COS) or oligochitosan has been found to induce plant defense response.In order to study the mechanism of COS induced tobacco defense response,nitric oxide(NO) generation was indentified in tobacco suspension cells induced by COS by using NO specific fluorescence diaminofluorescein diacetate(DAF-2DA) and Microplate Reader GEMINI EM.The results showed that tobacco cells treated with COS resulted in a rapid NO generation,whose fluorescence intensity was dependent of COS concentration.The NO generation was sensitive to NO scavenger Carboxy-PTIO potassium salt(cPTIO),NO synthase(NOS) inhibitor Nω-nitro-L-arginine methyl Ester(L-NAME),lanthanum chloride and ruthenium red.Nitrate reductase(NR) inhibitors sodium azide and sodium tungstate had no effect on NO generation induced by COS.L-NAME,cPTIO,EGTA,lanthanum chloride and ruthenium red reduced the expression of defense-related gene induced by COS.Above results showed that NOS mediated NO generation and Ca2+ participated in the process of COS-induced NO generation." @default.
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- W2378269469 date "2011-02-18" @default.
- W2378269469 modified "2023-09-27" @default.
- W2378269469 title "Nitric Oxide Signaling in Chitooligosaccharide Induced Tobacco Defense Response" @default.
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