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- W2378434315 abstract "Arabidopsis mutant hos10-1(for high expression of osmotically responsive gene) have reported,in which completely loss the capability of cold acclimation and more rapidly activated than in wild-type by low temperature or salt stress(NaCl).Microarray analysis revealed that At3g28220 genes with lower expression in the hos10-1 plant by 2-fold.In this study,RT-PCR,gene clone,transformation were used to study the function of At3g28220 gene in Arabidopsis.The result showed that At3g28220 gene can induced by cold acclimation and salt stress,the gene expression haven′t obviously distinction in different organs of Arabidopsis in different time.An At3g28220 ORF amplified by RT-PCR form Arabidopsis was norientation and inverted cloned into the downstream of 35S promoter of vector pCAMAIA2301.The construct was introduced in Arabidopsis through an Agrobacterium-mediated transformation.T1-generation plants screened by Kan resistance,confirmed by RT-PCR proved that At3g28220 gene had integrated into the Arabidopsis and expressed at the transcriptional level.In additon,we also found that transgenic plants had no significant growth inhibiton under normal growth condition.After a transient freezing treatment,transgenic plants had a significantly higher survival rate than of wild type plants.These results suggest that loss the capability of cold acclimation in mutant hos10-1 have the direct relation with the lower expression of the At3g28220 gene." @default.
- W2378434315 created "2016-06-24" @default.
- W2378434315 creator A5063899807 @default.
- W2378434315 date "2009-01-01" @default.
- W2378434315 modified "2023-09-23" @default.
- W2378434315 title "Analysis on Cold Tolerance of At3g28220 Gene in Arabidopsis" @default.
- W2378434315 hasPublicationYear "2009" @default.
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