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- W2378499932 abstract "Objective To explore functional role of PKD1 on the AP1 transcriptional activation and its targets gene in human HeLa cells.Methods pcDNA3.1,wild type of pcDNA-PKD1, kinase dead mutant of pcDNA-PKD1-DN,non specific siRNA (si-CTL) or siRNA of PKD1was cotransfected with activator protein 1(AP1)luciferase reporter AP1-luc and Renilla luciferase reporter pRL-SV40 into human HeLa cells.After 48 h transfection,cell lystates were harvested to measure the exogenous overexpression of wild type PKD1 and dominate negative PKD1 or endogenous PKD1 knockdown by siRNA using Western blot assay.Furthermore,AP1 transcriptional activity were determined by dual-luciferase reporter assay.Results Over-expression of PKD1,dominant negative mutant PKD1(PKD1-DN) or knockdown of PKD1 by siRNA in HeLa cells was confirmed by Western blot.Compared with pcDNA3.1,AP1 transcriptional activation triggered by PKD1 was significantly increased in HeLa cells transfected with pcDNA-PKD1(P0.05),whereas dramatically reduced by pcDNA-PKD1-DN transfection compared with wild type of pcDNA-PKD1 transfection(P0.05).In addition,AP1 transcriptional activation was also significantly inhibited by siRNA knockdown of endogenous PKD1 compared with control siRNA transfection(P0.001).Conclusion Enhanced AP1 transcriptional activation induced by PKD1 expression and kinase activity in HeLa cells suggested that PKD1 may regulate target genes expression through AP1 transcriptional activation." @default.
- W2378499932 created "2016-06-24" @default.
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- W2378499932 date "2010-01-01" @default.
- W2378499932 modified "2023-09-23" @default.
- W2378499932 title "PKD1 contributes to activator protein-1 activation in human HeLa cells" @default.
- W2378499932 hasPublicationYear "2010" @default.
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