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- W2378577560 abstract "Cardionatrin Ⅲ (CNⅢ ; 24AA), a small molecular peptide hapten, was cross-linked to bovine serum albumin (BSA) by glutaraldehyde two-step conjugation procedure. Anti-CN Ⅲ monoclonal antibody (CN Ⅲ -McAb) was prepared through immunization of BALB/c mice with purifed CN Ⅲ - BSA conjugate to get the mouse spleen cells sensitized and fuse them with SP2/0 myeloma cells, and at the same time CN Ⅲ - polyclonal antibody (CN Ⅲ - PcAb) was prepared by immunizing rabbits with the conjugate. The tilers of CN Ⅲ - McAb and CN Ⅲ -PcAb determined by ELISA-indirect method were 10-7 - 10-8 and 10-4-10-5, respectively. CN Ⅲ -McAb and CN Ⅲ - PcAb were separately coupled with CNBr-activated Sepharose 4B, the two immunosorbents and corresponding affinity chromatography columns were prepared and used for purification of CN Ⅲ in a sample. The CN Ⅲthus obtained showed significant eifects on blood pressure and urine volume of an experimental Wistar male rat. Our results also show a good immunogenicity of CN Ⅲ - albumin conjugate prepared by the method mentioned above." @default.
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- W2378577560 date "1992-01-01" @default.
- W2378577560 modified "2023-09-22" @default.
- W2378577560 title "Preparation of cardionatrin - albumin conjugate and its immunoassay" @default.
- W2378577560 hasPublicationYear "1992" @default.
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